Bacillus subtilis (natto) plasmid pLS20 mediates interspecies plasmid transfer

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Characterization of the replication region of the Bacillus subtilis plasmid pLS20: a novel type of replicon.

A 3.1 kb fragment of the large (approximately 55 kb) Bacillus subtilis plasmid pLS20 containing all the information for autonomous replication was cloned and sequenced. In contrast to the parental plasmid, derived minireplicons were unstably maintained. Using deletion analysis the fragment essential and sufficient for replication was delineated to 1.1 kb. This 1.1 kb fragment is located between...

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Characterization of the replication region of plasmid pLS32 from the Natto strain of Bacillus subtilis.

Plasmid pL32 from the Natto strain of Bacillus subtilis belongs to a group of low-copy-number plasmids in gram-positive bacteria that replicate via a theta mechanism of replication. We studied the DNA region encoding the replication protein, RepN, of pLS32, and obtained the following results. Transcription of the repN gene starts 167 nucleotides upstream from the translational start site of rep...

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Plasmid deletion formation in Bacillus subtilis.

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1987

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.169.11.5271-5278.1987